Molecular genetic analyses of the soybean mosaic virus NIa proteinase

Genetic analysis of resistance to soybean mosaic virus in j05 soybean.

Soybean cultivar J05 was identified to be resistant to the most virulent strain of soybean mosaic virus (SMV) in northeastern China. However, the reaction of J05 to SMV strains in the United States of America is unknown, and genetic information is needed to utilize this germplasm in a breeding program. The objectives of this study were to determine the reaction of J05 to all US strains of SMV (...

Potyviral NIa proteinase, a proteinase with novel deoxyribonuclease activity.

The NIa proteinase from pepper vein banding virus (PVBV) is a sequence-specific proteinase required for processing of viral polyprotein in the cytoplasm. It accumulates in the nucleus of the infected plant cell and forms inclusion bodies. The function of this protein in the nucleus is not clear. The purified recombinant NIa proteinase was active, and the mutation of the catalytic residues His-4...

The Nuclear Inclusion a (NIa) Protease of Turnip Mosaic Virus (TuMV) Cleaves Amyloid-β

BACKGROUND The nuclear inclusion a (NIa) protease of turnip mosaic virus (TuMV) is responsible for the processing of the viral polyprotein into functional proteins. NIa was previously shown to possess a relatively strict substrate specificity with a preference for Val-Xaa-His-Gln↓, with the scissile bond located after Gln. The presence of the same consensus sequence, Val(12)-His-His-Gln(15), ne...

Study of resistance to cucumber mosaic virus watermelon mosaic virus and zucchini yellow mosaic virus in melon cultivars

Chapter Two Biological and Molecular Diversity of Naturally Occurring Resistance Breaking Strains of Soybean mosaic virus

Resistance to Soybean mosaic virus (SMV; Genus Potyvirus; Family Potyviridae) is controlled by single dominant genes at three distinct loci, Rsv1, Rsv3 and Rsv4, in soybean (Glycine max [L.] Merr.). SMV isolates are classified into pathotypes G1 through G7 based on the differential reactions on resistant soybean cultivars. Most SMV isolated in the USA is of the G1 to G3 pathotypes. Since the cu...